Details

Autor(en) / Beteiligte

• Park, Min Cheol
• Hur, Jae Young
• Kwon, Keon Woo
• Park, Sang-Hyun
• Suh, Kahp Y

Titel

Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscus

Ist Teil von

• Lab on a chip, 2006-01, Vol.6 (8), p.988-994

Ort / Verlag

England

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• We present a simple cell docking method induced by receding meniscus to capture non-adherent yeast cells onto microwells inside a microfluidic channel. Microwells were fabricated either by capillary moulding of UV curable polyurethane acrylate (PUA) onto glass substrate or direct replica moulding of poly(dimethyl siloxane) (PDMS). A cell suspension of the budding yeast, Saccharomyces cerevisiae, was introduced into the microfluidic channel by surface tension driven capillary flow and a receding meniscus was subsequently generated by evaporation. As the meniscus progressed, one to multiple yeast cells were spontaneously captured onto microwells by lateral capillary force created at the bottom of the meniscus. Using this cell-based platform, we observed the response of yeast cells upon stimulation by a mating pheromone (alpha-factor) by monitoring the expression of green fluorescent protein (GFP) with time. It was observed that alpha-factor triggered the expression of GFP at 60 min after stimulation and the fluorescence intensity was sustained for an additional 60 min without changes.