Immunity (Cambridge, Mass.), 2006-07, Vol.25 (1), p.79-91
2006
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- Autor(en) / Beteiligte
- Titel
- The Tec Family Tyrosine Kinases Itk and Rlk Regulate the Development of Conventional CD8 + T Cells
- Ist Teil von
- Immunity (Cambridge, Mass.), 2006-07, Vol.25 (1), p.79-91
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The Tec family tyrosine kinases, Itk and Rlk, are expressed in thymocytes and peripheral T cells and regulate thresholds of T cell receptor signaling. Yet little is known about the specific role of Itk- and Rlk-dependent signals in CD8 + T cell maturation. We show here that Itk −/− and Rlk −/− Itk −/− mice were nearly devoid of conventional CD8 + T cells and, instead, contained a large population of CD8 + T cells that bear striking similarity to lineages of innate lymphocytes. Itk −/− and Rlk −/− Itk −/− CD8 + thymocytes and T cells were CD44 hi, CD122 +, and NK1.1 +; were able to produce interferon-γ directly ex vivo; and were dependent on interleukin-15. Itk −/− and Rlk −/− Itk −/− CD8 + thymocytes expressed abundant transcripts for the T box transcription factor, eomesodermin, correlating with their phenotype and function. These data indicate a critical role for Itk and Rlk in conventional CD8 + T cell development in the thymus.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1074-7613eISSN: 1097-4180DOI: 10.1016/j.immuni.2006.05.012Titel-ID: cdi_proquest_miscellaneous_68667027
- Format
- –
- Schlagworte
- Animals, Animals, Newborn, CD8-Positive T-Lymphocytes - cytology, CD8-Positive T-Lymphocytes - enzymology, CD8-Positive T-Lymphocytes - immunology, Cell Differentiation, Cell Proliferation, CELLIMMUNO, Cells, Cultured, DEVBIO, Gene expression, Genotype & phenotype, Hyaluronan Receptors - metabolism, Immunologic Memory - immunology, Interleukin-15 - deficiency, Interleukin-15 - genetics, Interleukin-15 - metabolism, Kinases, Lymphocyte Activation - immunology, Lymphocytes, Mice, Mice, Inbred C57BL, Mice, Knockout, Phenotype, Protein-Tyrosine Kinases - classification, Protein-Tyrosine Kinases - deficiency, Protein-Tyrosine Kinases - genetics, Protein-Tyrosine Kinases - metabolism, Receptors, Antigen, T-Cell - metabolism, SIGNALING, Studies, T cell receptors, T-Box Domain Proteins - metabolism, Thymus Gland - immunology, Thymus Gland - metabolism, Time Factors, Up-Regulation, Xenopus Proteins - metabolism