Details

Autor(en) / Beteiligte

• Herren, Christopher D
• Mitra, Arindam
• Palaniyandi, Senthil Kumar
• Coleman, Adam
• Elankumaran, Subbiah
• Mukhopadhyay, Suman

Titel

BarA-UvrY Two-Component System Regulates Virulence in Avian Pathogenic Escherichia coli O78:K80:H9

Ist Teil von

• Infection and Immunity, 2006-08, Vol.74 (8), p.4900-4909

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• The BarA-UvrY two-component system (TCS) in Escherichia coli is known to regulate a number of phenotypic traits. Both in vitro and in vivo assays, including the chicken embryo lethality assay, showed that this TCS regulates virulence in avian pathogenic E. coli (APEC) serotype O78:K80:H9. A number of virulence determinants, such as the abilities to adhere, invade, persist within tissues, survive within macrophages, and resist bactericidal effects of serum complement, were compromised in mutants lacking either the barA or uvrY gene. The reduced virulence was attributed to down regulation of type 1 and Pap fimbriae, reduced exopolysaccharide production, and increased susceptibility to oxidative stress. Our results indicate that BarA-UvrY regulates virulence properties in APEC and that the chicken embryo lethality assay can be used as a surrogate model to determine virulence determinants and their regulation in APEC strains.