Details

Autor(en) / Beteiligte

• MAREE, A. O.
• CURTIN, R. J.
• CHUBB, A.
• DOLAN, C.
• COX, D.
• O'BRIEN, J.
• CREAN, P.
• SHIELDS, D. C.
• FITZGERALD, D. J.

Titel

Cyclooxygenase‐1 haplotype modulates platelet response to aspirin

Ist Teil von

• Journal of thrombosis and haemostasis, 2005-10, Vol.3 (10), p.2340-2345

Ort / Verlag

Oxford, UK: Blackwell Science Inc

Erscheinungsjahr

2005

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Background: Aspirin (acetylsalicylic acid) irreversibly inhibits platelet cyclooxygenase (COX)‐1, the enzyme that converts arachidonic acid (AA) to the potent platelet agonist thromboxane (TX) A2. Despite clear benefit from aspirin in patients with cardiovascular disease (CAD), evidence of heterogeneity in the way individuals respond has given rise to the concept of ‘aspirin resistance.’Aims: To evaluate the hypothesis that incomplete suppression of platelet COX as a consequence of variation in the COX‐1 gene may affect aspirin response and thus contribute to aspirin resistance. Patients and methods: Aspirin response, determined by serum TXB2 levels and AA‐induced platelet aggregation, was prospectively studied in patients (n = 144) with stable CAD taking aspirin (75–300 mg). Patients were genotyped for five single nucleotide polymorphisms in COX‐1 [A‐842G, C22T (R8W), G128A (Q41Q), C644A (G213G) and C714A (L237M)]. Haplotype frequencies and effect of haplotype on two platelet phenotypes were estimated by maximum likelihood. The four most common haplotypes were considered separately and less common haplotypes pooled. Results: COX‐1 haplotype was significantly associated with aspirin response determined by AA‐induced platelet aggregation (P = 0.004; 4 d.f.). Serum TXB2 generation was also related to genotype (P = 0.02; 4 d.f.). Conclusion: Genetic variability in COX‐1 appears to modulate both AA‐induced platelet aggregation and thromboxane generation. Heterogeneity in the way patients respond to aspirin may in part reflect variation in COX‐1 genotype.