Details

Autor(en) / Beteiligte

• Marrs, Glen S
• Honda, Takashi
• Fuller, Leah
• Thangavel, Ramasamy
• Balsamo, Janne
• Lilien, Jack
• Dailey, Michael E
• Arregui, Carlos

Titel

Dendritic arbors of developing retinal ganglion cells are stabilized by beta 1-integrins

Ist Teil von

• Molecular and cellular neuroscience, 2006-07, Vol.32 (3), p.230-241

Ort / Verlag

United States

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• The architecture of dendritic arbors is a defining characteristic of neurons and is established through a sequential but overlapping series of events involving process outgrowth and branching, stabilization of the global pattern, and synapse formation. To investigate the roles of cadherins and beta1-integrins in maintaining the global architecture of the arbor, we used membrane permeable peptides and transfection with dominant-negative constructs to disrupt adhesion molecule function in intact chick neural retina at a stage when the architecture of the ganglion cell (RGC) arbor is established but synapse formation is just beginning. Inactivation of beta1-integrins induces rapid dendrite retraction, with loss of dynamic terminal filopodia followed by resorption of major branches. Disruption of N-cadherin-beta-catenin interactions has no effect; however, dendrites do retract following perturbation of the juxtamembrane region of N-cadherin, which disrupts N-cadherin-mediated adhesion and initiates a beta1-integrin inactivating signal. Thus, developing RGC dendritic arbors are stabilized by beta1-integrin-dependent processes.