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CD45-positive cells of haematopoietic origin enhance chondrogenic marker gene expression in rat marrow stromal cells
Ist Teil von
International journal of molecular medicine, 2006-08, Vol.18 (2), p.233-240
Ort / Verlag
Greece: D.A. Spandidos
Erscheinungsjahr
2006
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Adult mesenchymal stem cells (MSCs) can be readily isolated from bone marrow,
expanded in culture and subsequently subjected to differentiation into various
connective tissue lineages. In general, for animal studies separation of MSCs
from other bone marrow-derived cells is achieved by sole adherence to plastic
surface of tissue culture flasks; however, this procedure produces a heterogeneous
cell population containing CD45-positive haematopoietic cells (HCs) and haematopoietic
stem cells (HSCs). It is known, that mixed cell cultures consisting of cocultures
of differentiated somatic cells with adult stem cells promote differentiation
towards specific cell lineages. For determining the effect of the CD45-positive
cell population on the differentiation potential of MSCs, we sorted out the bone
marrow-derived adherent cells by immunomagnetic technique (MACS™) to attain a
subpopulation of CD45-depleted cells. Herein, we show that the presence of adherent
CD45-positive HCs not only promote expression of the chondrogenic marker genes
Col2a1, COMP and Sox9, but also of Col1a1, Col10a1 and to a certain degree Cbfa1
in MSCs when cultured in an appropriate three-dimensional environment. These observations
constitute a step towards unravelling the influence of haematopoietic cells on
chondrogenic differentiation of MSCs.