Details

Autor(en) / Beteiligte

• Schüpbach, Jörg
• Tomasik, Zuzana
• Knuchel, Marlyse
• Opravil, Milos
• Günthard, Huldrych F.
• Nadal, David
• Böni, Jürg

Titel

Optimized virus disruption improves detection of HIV-1 p24 in particles and uncovers a p24 reactivity in patients with undetectable HIV-1 RNA under long-term HAART

Ist Teil von

• Journal of medical virology, 2006-08, Vol.78 (8), p.1003-1010

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• HIV‐1 p24 antigen (p24) measurement by signal amplification‐boosted ELISA of heat‐denatured plasma is being evaluated as an alternative to HIV‐1 RNA quantitation in resource‐poor settings. Some observations suggested that virion‐associated p24 is suboptimally detected using Triton X‐100‐based virus dissociation buffer (kit buffer). A new reagent (SNCR buffer) containing both denaturing and non‐denaturing detergents was therefore developed and evaluated. The SNCR buffer increased the measured p24 concentration about 1.5‐ to 3‐fold in HIV‐negative plasma reconstituted with purified HIV‐1 particles, while not increasing the background. Among 127 samples of HIV‐1‐positive patients with moderate to high concentrations of HIV‐1 RNA the increase was about threefold across the entire concentration range (P < 0.0001). Specificity before neutralization among prospectively tested clinical samples ruled HIV‐negative was 828 of 845 (98.0%) for the SNCR buffer and 464 of 479 (96.9%) for kit buffer. Specificity after confirmatory neutralization of reactive samples or a follow‐up test was 100% with either buffer. Surprisingly, the SNCR buffer revealed a p24 reactivity in 115 of 187 samples (61.5%) from adult patients exhibiting undetectable HIV‐1 RNA below 5 copies/ml for a duration of 6–30 months under HAART (3.7% with kit buffer). The rate of p24 reactivity in these patients did not decrease with duration of HAART. In conclusion, the SNCR buffer improves the detection of particle‐associated HIV‐1 p24, thereby increasing the measured p24 concentration in samples with medium to high HIV‐1 RNA. It also uncovers the presence of a p24 reactivity, whose identity remains to be determined, in a significant fraction of samples with undetectable HIV‐1 RNA under long‐term HAART. J. Med. Virol. 78:1003–1010, 2006. © 2006 Wiley‐Liss, Inc.