Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
In search of compounds which show tumor-specific cytotoxic activity, two 3,5-dibenzoyl-1,4-dihydropyridines ( GB5 , GB12 ) were found to show one or two orders higher cytotoxic activity against human tumor cell lines (squamous cell carcinoma HSC-2,
HSC-3, submandibular gland carcinoma HSG, promyelocytic leukemia HL-60) than human normal cells (gingival fibroblast HGF,
pulp cells HPC, periodontal ligament fibroblasts HPLF). GB5 and GB12 weakly induced several apoptosis-associated properties, such as inter-nucleosomal DNA fragmentation, and activation of caspases
-3, -8 and -9, in both HL-60 and HSC-2 cells. Western blot analysis showed that GB5 and GB12 transiently increased the expression of both anti-apoptotic protein (Bcl-2) and proapoptotic proteins (Bax and Bad) in HL-60
cells. ESR spectroscopy showed these compounds did not produce any detectable amount of radicals, nor scavenged superoxide
(generated by hypoxanthine-xanthine oxidase reaction) or nitric oxide (generated by 1-hydroxy-2-oxo-3-(N-3-methyl-3-aminopropyl)-3-methyl-1-triazene),
suggesting that the induction of cytotoxic action is not via a radical-mediated reaction. The present study suggests that
GB5 and GB12 may induce non-apoptotic cell death in tumor cell lines.