Details

Autor(en) / Beteiligte

• Lichterfeld, Mathias
• Williams, Katie L.
• Mui, Stanley K.
• Shah, Shivani S.
• Mothe, Bianca R.
• Sette, Alessandro
• Kim, Arthur
• Johnston, Mary N.
• Burgett, Nicole
• Frahm, Nicole
• Cohen, Daniel
• Brander, Christian
• Rosenberg, Eric S.
• Walker, Bruce D.
• Altfeld, Marcus
• Yu, Xu G.

Titel

T cell receptor cross-recognition of an HIV-1 CD8+ T cell epitope presented by closely related alleles from the HLA-A3 superfamily

Ist Teil von

• International immunology, 2006-07, Vol.18 (7), p.1179-1188

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2006

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• HLA-A3 and -A11 share similar peptide-binding motifs, however, it is unclear if promiscuous epitope presentation by HLA-A3 or HLA-A11 is associated with promiscuous TCR recognition. Here, we show that despite widespread cross-presentation of identical HIV-1 peptides in HIV-1-infected individuals expressing HLA-A3 or HLA-A11, peptides presented by HLA-A3 or HLA-A11 commonly exhibited clear immune distinctiveness with exclusive TCR recognition. Yet, using HLA-A3 and HLA-A11 tetramers for testing T cell cross-recognition of the HIV-1 Nef QK10 epitope, we observed in two study persons that specific CD8+ T cell populations were able to cross-recognize this peptide in the context of both HLA-A3 and HLA-A11. This cross-recognition was mediated by single cross-reactive TCRs, as shown by TCR sequencing in conjunction with TCR Vβ chain immunostaining. In each cross-reactive cell population, multiple TCR β chain variants were detected in the presence of only one TCR α chain variant. Thus, despite distinct TCR recognition of HLA-A3 or HLA-A11 presented HIV-1 peptides in the vast majority of cases, specific TCRs can cross-recognize their antigen in the context of both HLA-A3 and HLA-A11.