Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Gene Expression Profiles Associated with Response to Chemotherapy in Epithelial Ovarian Cancers
Ist Teil von
Clinical cancer research, 2005-09, Vol.11 (17), p.6300-6310
Ort / Verlag
Philadelphia, PA: American Association for Cancer Research
Erscheinungsjahr
2005
Quelle
MEDLINE
Beschreibungen/Notizen
Purpose: The goal of this study was to determine whether distinct gene expression profiles are associated with intrinsic and/or acquired
chemoresistance in epithelial ovarian carcinoma.
Experimental Design: Gene expression profiles were generated from 21 primary chemosensitive tumors and 24 primary chemoresistant tumors using
cDNA-based microarrays. Gene expression profiles of both groups of primary tumors were then compared with those of 15 ovarian
carcinomas obtained following platinum-based chemotherapy (“postchemotherapy” tumors). A theme discovery tool was used to
identify functional categories of genes involved in drug resistance.
Results: Comparison of primary chemosensitive and chemoresistant tumors revealed differential expression of 85 genes ( P < 0.001). Comparison of gene expression profiles of primary chemosensitive tumors and postchemotherapy tumors revealed more
robust differences with 760 genes differentiating the two groups ( P < 0.001). In contrast, only 230 genes were differentially expressed between primary chemoresistant and postchemotherapy groups
( P < 0.001). Common to both gene lists were 178 genes representing transcripts differentially expressed between postchemotherapy
tumors and all primary tumors irrespective of intrinsic chemosensitivity. The gene expression profile of postchemotherapy
tumors compared with that of primary tumors revealed statistically significant overrepresentation of genes encoding extracellular
matrix–related proteins.
Conclusions: These data show that gene expression profiling can discriminate primary chemoresistant from primary chemosensitive ovarian
cancers. Gene expression profiles were also identified that correlate with states of intrinsic and acquired chemoresistance
and that represent targets for future investigation and potential therapeutic interventions.