Zhong hua yi xue za zhi, 2007-08, Vol.87 (32), p.2285-2288
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Effect of calpain 1 on structural remodeling and contractile dysfunction in atrial fibrillation: experiment with dogs
- Ist Teil von
- Zhong hua yi xue za zhi, 2007-08, Vol.87 (32), p.2285-2288
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To test the causal relationship between calpain activation and atrial structural changes during atrial fibrillation (AF). The tip of a spiral mono-polar pacing lead was fixed to the right atrial appendages of 15 dogs randomly divided into 3 equal groups: calpain inhibitor group, undergoing continuous pacing with the impulse of 600 beats/min for 3 weeks and intravenous injection of N-acetyl-Leu-Leu-Met (ALLM), a calpain inhibitor for 3 weeks; control group, undergoing continuous pacing and intravenous injection of dimethyl sulfoxide (DMSO; and sham operation group, given DMSO injection without pacing. Ultrasonography was used to observe the changes of the structures of left atrium and left atrial appendage and the heart function as well. Specimens of atrial muscles were obtained. Calpain 1 activity was detected by Suc-Leu-Leu-Val-Tyr-7-amino-4-methyl-coumarin method. HE staining was conducted to observe the myolysis. Western blotting was used to detect the protein expression of troponin I (TnI) and myosin. The
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0376-2491Titel-ID: cdi_proquest_miscellaneous_68507853
- Format
- –
- Schlagworte
- Animals, Atrial Fibrillation - metabolism, Atrial Fibrillation - physiopathology, Blotting, Western, Calpain - metabolism, Disease Models, Animal, Dogs, Heart - drug effects, Heart - physiopathology, Injections, Intravenous, Myocardial Contraction, Myocardium - metabolism, Myosins - metabolism, Oligopeptides - administration & dosage, Oligopeptides - pharmacology, Random Allocation