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Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand
Ist Teil von
Journal of Chromatography A, 2005-08, Vol.1084 (1), p.108-112
Ort / Verlag
Amsterdam: Elsevier B.V
Erscheinungsjahr
2005
Link zum Volltext
Quelle
Access via ScienceDirect (Elsevier)
Beschreibungen/Notizen
Stationary phase containing quinolin-8-ol immobilized on macroporous methacrylate support for the affinity chromatography of porcine pepsin A is described. Optimized chromatographic conditions for separation of porcine pepsin A on this stationary phase were found investigating the influence of pH, concentration, ionic strength and chemical composition of the used mobile phases. The stationary phase shows a good reproducibility of chromatographic analyses (relative standard deviation, ±2%), a high recovery (ca. 93%) and a satisfactory capacity (13
mg pepsin A/1
mL stationary phase) for porcine pepsin A. The obtained findings confirm the applicability of affinity chromatography on the stationary phase with immobilized quinolin-8-ol to the isolation and determination of porcine pepsin A.