Details

Autor(en) / Beteiligte

• Sulová, Zdena
• Orlický, Jozef
• Fiala, Roderik
• Dovinová, Ima
• Uhrík, Branislav
• Seres, Mário
• Gibalová, Lenka
• Breier, Albert

Titel

Expression of P-glycoprotein in L1210 cells is linked with rise in sensitivity to Ca2

Ist Teil von

• Biochemical and biophysical research communications, 2005-09, Vol.335 (3), p.777-784

Ort / Verlag

United States

Erscheinungsjahr

2005

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• L1210/VCR cell line (R) was obtained by adaptation of the L1210 mouse leukaemia cells (S) to vincristine and showed P-glycoprotein (P-gp) mediated multidrug resistance (MDR). R cells were observed to be more sensitive to high external calcium as parental S. More pronounced calcium uptake was observed for R cells. Moreover, differences in intracellular calcium cell localization between S and R cells were found ultrastructurally following a calcium precipitating cytochemical method. In S cells, calcium precipitates were found to be localized predominantly along the cell surface coat and within mitochondria delineating the cristae. In R cells, precipitates were also found inside nuclei, at the border of heterochromatin clumps, and scattered within the cytoplasm. High extracellular calcium did not influence the P-gp mediated extrusion of calcein/AM as P-gp substrate. These results indicate that calcium enters and consequently damages the MDR cells to a higher extent than parental cells.