Details

Autor(en) / Beteiligte

• Faria, Adriana F.
• de Souza, Marcus V.N.
• de Almeida, Mauro V.
• de Oliveira, Marcone A.L.

Titel

Simultaneous separation of five fluoroquinolone antibiotics by capillary zone electrophoresis

Ist Teil von

• Analytica chimica acta, 2006-10, Vol.579 (2), p.185-192

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A novel methodology has been developed for simultaneous separation of ciprofloxacin (CPFLX), gatifloxacin (GTFLX), levofloxacin (LVFLX), moxifloxacin (MFLX) and sparfloxacin (SPFLX) fluoroquinolone antibiotics (FQs), using capillary zone electrophoresis (CZE) with UV detection at 282 nm. Electrolyte composition was optimized through the variation of the Tris/hydrochloride and sodium tetraborate buffer mixture. The electrolyte consisted of a 25 mmol L −1 Tris/hydrochloride and 15 mmol L −1 sodium tetraborate buffer mixture resulting in pH 8.87. All analytes were separated in less than 3 min. The proposed method was applied to the separation of FQs in pharmaceutical formulations, and the assay results were within 95–105% of the label claim.