Zhong hua yi xue za zhi, 2007-07, Vol.87 (25), p.1773-1777
2007
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- Autor(en) / Beteiligte
- Titel
- Inhibition of the aquaporin-1 gene expression by RNA interference: experiment with cultured rat pleural mesothelial cells
- Ist Teil von
- Zhong hua yi xue za zhi, 2007-07, Vol.87 (25), p.1773-1777
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To investigate the influence of RNA interference (RNAi) on the expression of aquaporin-1 (AQP1) gene and to investigate the feasibility of gene therapy for pleural effusion. Two recombinant plasmids with shRNAs targeting the AQP1 gene, AQP1-1-pGenesil and AQP1-2-pGenesil-1 were constructed. Pleural mesothelial cells were obtained from rats, cultured, and randomly divided into 5 groups: blank control group, Lipofectamine 2000 control group, HK negative control group, AQP1-1-pGenesil-1 transfected group, and AQP1-2-pGenesil-1 transfected group. RT-PCR and Western blotting were used to detect the mRNA and protein expression of AQP1. The mRNA expression levels of aquaporin-1 of the AQP1-1-pGenesil-1 and AQP1-2-pGenesil-1 transfected groups were inhibited by 83.5% and 90.9% respectively, and the protein expression levels of the AQP1-1-pGenesil-1 and AQP1-2-pGenesil-1 transfected groups were inhibited by 41.2% and 67.6% respectively. RNAi can successfully inhibit the expression of AQP1 and has the feature of sequen
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0376-2491Titel-ID: cdi_proquest_miscellaneous_68358266
- Format
- –
- Schlagworte
- Animals, Aquaporin 1 - genetics, Aquaporin 1 - metabolism, Blotting, Western, Cells, Cultured, Epithelial Cells - cytology, Epithelial Cells - metabolism, Flow Cytometry, Male, Pleura - cytology, Random Allocation, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference, RNA, Messenger - genetics, RNA, Messenger - metabolism, RNA, Small Interfering - genetics, Transfection