Leukemia research, 2007-01, Vol.31 (1), p.75-82
2007
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study
- Ist Teil von
- Leukemia research, 2007-01, Vol.31 (1), p.75-82
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0145-2126eISSN: 1873-5835DOI: 10.1016/j.leukres.2006.02.026Titel-ID: cdi_proquest_miscellaneous_68352699
- Format
- –
- Schlagworte
- Anticarcinogenic Agents - pharmacology, Cell cycle arrest, Cell Division - drug effects, Electrophoresis, Gel, Two-Dimensional, Enzyme Inhibitors - pharmacology, Genistein, Genistein - pharmacology, Hematology, Oncology and Palliative Medicine, HL-60 Cells, Humans, Kinetics, Leukemia HL-60, Mass Spectrometry, Neoplasm Proteins - genetics, Neoplasm Proteins - isolation & purification, Protein-Tyrosine Kinases - antagonists & inhibitors, Proteomics, Reproducibility of Results, Ribonucleoproteins - genetics, Ribonucleoproteins - isolation & purification, Trypsin, Tyrosine kinase