Chung-hua fu chʿan kʿo tsa chih, 2006-12, Vol.41 (12), p.803-805
2006
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- Autor(en) / Beteiligte
- Titel
- Study of altered expression of annexin IV and human endometrial receptivity
- Ist Teil von
- Chung-hua fu chʿan kʿo tsa chih, 2006-12, Vol.41 (12), p.803-805
- Ort / Verlag
- China
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To explore the association of altered expression of annexin IV in human endometrium during the implantation window and endometrial receptivity. A comparative proteomic strategy, in a combination of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), was adopted to search for proteome alternations of pre-receptive (day LH + 2) versus receptive (LH + 7) endometria. The location and abundance of the identified differentially expressed protein- annexin IV were analyzed by immunostaining and western blot. By comparing protein profiles of LH + 2 and LH + 7 samples, we found a protein up-regulated 2.12 times in LH + 7 samples, with a relative molecular weight of 36,000 and an isoelectric point near pH 5.8. It was characterized using mass spectrometry and was identified as annexin IV. Immunohistochemical analysis revealed an altered localization of annexin IV--in the epithelia on day LH + 2, and both in
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0529-567XTitel-ID: cdi_proquest_miscellaneous_68323713
- Format
- –
- Schlagworte
- Adult, Annexin A4 - biosynthesis, Blotting, Western, Electrophoresis, Gel, Two-Dimensional - methods, Embryo Implantation - physiology, Endometrium - metabolism, Female, Humans, Immunohistochemistry, Menstrual Cycle - physiology, Pregnancy, Proteomics, Receptors, Estrogen - metabolism, Receptors, Lymphocyte Homing - metabolism, Receptors, Progesterone - metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods, Time Factors