Details

Autor(en) / Beteiligte

• Dai, Shu-zhen
• Kong, Shou-fang
• Huang, Li-li
• Luo, Bing

Titel

Construction and package of the expression plasmid pAdEasy-1 system encoding the human papillomavirus 16 E7 gene and the gene's influence on HeLa cells

Ist Teil von

• Chung-hua fu chʿan kʿo tsa chih, 2006-09, Vol.41 (9), p.612-617

Ort / Verlag

China

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effect of human papillomavirus 16 E7 gene on cell cycle of cervical cancer HeLa cell, through construction and expression of human papillomavirus 16 E7 gene with adenovirus vector. Recombinant adenovirus which expressed E7 gene was constructed and packed. Flow cytometry (FCM) was used to detect the changes of cell cycle phase and cyclin D1 between cells infected and uninfected by recombinant adenovirus. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphennyltetrazolium bromide (MTT) colorimetric assay was used in the detection of the alteration of cell growth. The recombinant adenovirus had stable efficiency of infection and E7 genes could be expressed stably. The result of MTT showed the multiplication of HeLa cells was accelerated from 0.27 +/- 0.03 before infection to 0.38 +/- 0.02 after infection (P < 0.01). FCM showed the number of cells in S phase increased from (26.0 +/- 0.4)% to (36.0 +/- 2.0)% at 12 hours and (49.9 +/- 4.2)% at 24 hours after infection (P < 0.05). Cyclin D1 expression was