Details

Autor(en) / Beteiligte

• Nollens, H.H
• Green, L.G
• Duke, D
• Walsh, M.T
• Chittick, B
• Gearhart, S
• Klein, P.A
• Jacobson, E.R

Titel

Development and validation of monoclonal and polyclonal antibodies for the detection of immunoglobulin G of bottlenose dolphins (Tursiops truncatus)

Ist Teil von

• Journal of veterinary diagnostic investigation, 2007-09, Vol.19 (5), p.465-470

Ort / Verlag

Los Angeles, CA: J Vet Diagn Invest

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Correspondence: 1 Corresponding Author: Hendrik Nollens, Marine Mammal Health Program, SACS, College of Veterinary Medicine, University of Florida, PO Box 100126, Gainesville, FL 32610, e-mail: NollensH{at}vetmed.ufl.edu Antibodies directed against species-specific immunoglobulin G (IgG) have a broad range of applications in serologic and immunologic research and in the development of clinical assays. Validated anti-IgG antibodies for marine mammal species are in short supply. The objective of this study was to produce and validate antibodies with specificity for IgG of the common bottlenose dolphin ( Tursiops truncatus ). Bottlenose dolphin IgG was purified using protein G. Two mouse monoclonal antibodies and a rabbit polyclonal antibody were developed from mice and rabbits immunized with bottlenose dolphin IgG. The specificity of the monoclonal antibodies and the polyclonal antibody for bottlenose dolphin IgG was first verified by Western blot analysis and enzyme-linked immunosorbent assay (ELISA). For further validation, both monoclonal antibodies and the polyclonal antibody were incorporated in an indirect ELISA for the detection of the immune response of bottlenose dolphins to a vaccine antigen. Three bottlenose dolphins were immunized with a commercial Erysipelothrix rhusiopathiae vaccine, and serial blood samples were collected from all dolphins for measurement of levels of circulating antibodies. Seroconversion was observed in all 3 dolphins by use of both monoclonal antibodies and the polyclonal antibody. Circulating antibodies were detectable as early as 6 days after immunization in 1 dolphin. Peak antibody levels were detected 14 days after the immunization. The ability to detect seroconversion in all 3 immunized bottlenose dolphins firmly establishes the specificity of the monoclonal antibodies and the polyclonal antibody for IgG of the common bottlenose dolphin. Key Words: Antibody • dolphin • immunoglobulin • monoclonal • polyclonal • serology • Tursiops