Details

Autor(en) / Beteiligte

• Chudakov, Dmitriy M
• Shcherbo, Dmitry
• Merzlyak, Ekaterina M
• Chepurnykh, Tatiana V
• Fradkov, Arkady F
• Ermakova, Galina V
• Solovieva, Elena A
• Lukyanov, Konstantin A
• Bogdanova, Ekaterina A
• Zaraisky, Andrey G
• Lukyanov, Sergey

Titel

Bright far-red fluorescent protein for whole-body imaging

Ist Teil von

• Nature methods, 2007-09, Vol.4 (9), p.741-746

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.