Science (American Association for the Advancement of Science), 2006-12, Vol.314 (5804), p.1464-1467
2006
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria
- Ist Teil von
- Science (American Association for the Advancement of Science), 2006-12, Vol.314 (5804), p.1464-1467
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2006
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Gene inventory and metagenomic techniques have allowed rapid exploration of bacterial diversity and the potential physiologies present within microbial communities. However, it remains nontrivial to discover the identities of environmental bacteria carrying two or more genes of interest. We have used microfluidic digital polymerase chain reaction (PCR) to amplify and analyze multiple, different genes obtained from single bacterial cells harvested from nature. A gene encoding a key enzyme involved in the mutualistic symbiosis occurring between termites and their gut microbiota was used as an experimental hook to discover the previously unknown ribosomal RNA-based species identity of several symbionts. The ability to systematically identify bacteria carrying a particular gene and to link any two or more genes of interest to single species residing in complex ecosystems opens up new opportunities for research on the environment.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1131370Titel-ID: cdi_proquest_miscellaneous_68206505
- Format
- –
- Schlagworte
- Animals, Bacteria, Bacteria - classification, Bacteria - genetics, Bacteriological methods and techniques used in bacteriology, Bacteriology, Biological and medical sciences, Biotechnology, Cell aggregates, Cells, Digestive system, Digestive System - microbiology, DNA, Ribosomal - genetics, Enzymes, Fluorescent Dyes, Formate-Tetrahydrofolate Ligase - genetics, Fundamental and applied biological sciences. Psychology, Genes, Genes, Bacterial, Genes, rRNA, Genetic engineering, Genetic technics, Genomes, Hindgut, In vitro gene amplification. Pcr technique, Insects, Isoptera, Isoptera - microbiology, Methods. Procedures. Technologies, Microbiology, Microfluidic Analytical Techniques, Microfluidics, Phylogenetics, Phylogeny, Polymerase chain reaction, Polymerase Chain Reaction - methods, Product category rules, Ribotyping, RNA, Ribosomal, 16S - genetics, rRNA genes, Spirochaetales - classification, Spirochaetales - genetics, Spirochaetales - metabolism, Symbiosis, Termites, Treponema - classification, Treponema - genetics, Treponema - metabolism