Details

Autor(en) / Beteiligte

• Takamori, Shigeo
• Holt, Matthew
• Stenius, Katinka
• Lemke, Edward A.
• Grønborg, Mads
• Riedel, Dietmar
• Urlaub, Henning
• Schenck, Stephan
• Brügger, Britta
• Ringler, Philippe
• Müller, Shirley A.
• Rammner, Burkhard
• Gräter, Frauke
• Hub, Jochen S.
• De Groot, Bert L.
• Mieskes, Gottfried
• Moriyama, Yoshinori
• Klingauf, Jürgen
• Grubmüller, Helmut
• Heuser, John
• Wieland, Felix
• Jahn, Reinhard

Titel

Molecular Anatomy of a Trafficking Organelle

Ist Teil von

• Cell, 2006-11, Vol.127 (4), p.831-846

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.