Details

Autor(en) / Beteiligte

• Ordway, Diane
• Palanisamy, Gopinath
• Henao-Tamayo, Marcela
• Smith, Erin E
• Shanley, Crystal
• Orme, Ian M
• Basaraba, Randall J

Titel

The Cellular Immune Response to Mycobacterium tuberculosis Infection in the Guinea Pig

Ist Teil von

• Journal of Immunology, 2007-08, Vol.179 (4), p.2532-2541

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

2007

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Pulmonary tuberculosis in guinea pigs is an extremely useful model for drug and vaccine testing due to the fact that its pathological disease process is similar to that present in humans. Progress in this field has been hindered because the tools necessary to undertake a complete immunological analysis of the guinea pig cellular immune response against Mycobacterium tuberculosis have been lacking. In this study, we combined a new flow cytometric gating strategy with immunohistochemistry to track T cells, B cells, and the MIL4 Ab, which detects both guinea pig heterophils (neutrophils) and eosinophils, to provide the first documentation of the kinetics of influx and positioning of these cell populations. The results show that the responding T cells are mostly CD4 cells and that after day 30 of the infection numbers of these cells in the lungs drops dramatically. These appear to be replaced by a steady increase in B cells and granulocytes which was associated with worsening lung pathology. These data reveal new information about the cellular phenotypes which mediate protective immunity or host immunopathogenesis during M. tuberculosis infection in this key animal model.