Details

Autor(en) / Beteiligte

• Jasper, Paul J.
• Rhee, Ki‐Jong
• Kalis, Susan L.
• Sethupathi, Periannan
• Yam, Pi‐Chen
• Zhai, Shi‐Kang
• Knight, Katherine L.

Titel

B lymphocyte deficiency in IgH‐transgenic rabbits

Ist Teil von

• European Journal of Immunology, 2007-08, Vol.37 (8), p.2290-2299

Ort / Verlag

Weinheim: WILEY‐VCH Verlag

Erscheinungsjahr

2007

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• We developed IgH‐transgenic rabbits carrying a productive VDJ‐Cμ Tg and found the rabbits were B cell‐deficient, with a 50–100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ‐Cμ Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg μ‐chain paired with VpreB and λ5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous μ‐chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle‐associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle‐associated epithelium, possibly through contact with intestinal microbiota. These IgH‐Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.