Details

Autor(en) / Beteiligte

• Vogelstein, Bert
• Diehl, Frank
• Li, Meng
• He, Yiping
• Kinzler, Kenneth W
• Dressman, Devin

Titel

BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions

Ist Teil von

• Nature methods, 2006-07, Vol.3 (7), p.551-559

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• BEAMing (beads, emulsions, amplification and magnetics) is a process built on emulsion PCR that (i) includes beads within the compartments and (ii) ensures that one strand of the PCR product is bound to the beads. Beads obtained via BEAMing accurately reflect the DNA diversity present in template populations and this method can be used to determine what fraction of a DNA population contains a specific mutation.