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Rho-kinase inhibitor, Y-27632, has an antinociceptive effect in mice
Ist Teil von
European journal of pharmacology, 2006-07, Vol.541 (1), p.49-52
Ort / Verlag
Amsterdam: Elsevier B.V
Erscheinungsjahr
2006
Quelle
MEDLINE
Beschreibungen/Notizen
The possible antinociceptive effect of a Rho-kinase inhibitor, (+)-(
R)-
trans-4-(1-aminoethyl)-
N-(4-pyridyl) cyclohexanecarboxamide dihydrochloride monohydrate (Y-27632), was investigated in mice by using the hot-plate and abdominal constriction response (writhing) tests. In addition, the expression of Rho-kinase protein (ROCK-2) was studied in the mouse brain and spinal cord by Western blotting. Male
balb/
c mice (
n
=
8, for each group) were used in the experiment. Hot-plate latency and the number of writhes were recorded in control and in Y-27632-treated (1–5 mg/kg, i.p.) groups. Y-27632 (1 mg/kg) did not affect hot-plate latency; however, it considerably diminished the number of writhes, from 89
±
12 in control to 30
±
6 in the mice treated with 1 mg/kg Y-27632 (
P
=
0.001). At a higher dose (5 mg/kg), Y-27632 prolonged the hot-plate latency from 8.7
±
1.0 s to 14.4
±
1.7 s (
P
=
0.005) and decreased the number of writhes from 80
±
8 to 24
±
7 (
P
=
0.002). Western blot analysis revealed that mouse spinal cord and brain homogenates expressed ROCK-2 protein. These results indicate that Rho-kinase may be involved in nociception and that its inhibitors, such as Y-27632, may represent a new type of antinociceptive drug.