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Data Requirements for Protein Identification Using Chemically-Assisted Fragmentation and Tandem Mass Spectrometry
Ist Teil von
European journal of mass spectrometry (Chichester, England), 2005-01, Vol.11 (2), p.161-167
Ort / Verlag
London, England: SAGE Publications
Erscheinungsjahr
2005
Quelle
MEDLINE
Beschreibungen/Notizen
Many laboratories identify proteins by searching tandem mass spectrometry data against genomic or protein sequence databases. These database searches typically use the measured peptide masses or the derived peptide sequence and, in this paper, we focus on the latter. We study the minimum peptide sequence data requirements for definitive protein identification from protein sequence databases. Accurate mass measurements are not needed for definitive protein identification, even when a limited amount of sequence data is available for searching. This information has implications for the mass spectrometry performance (and cost), data base search strategies and proteomics research.