Oral oncology, 2005-07, Vol.41 (6), p.607-613
2005
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- Autor(en) / Beteiligte
- Titel
- Differential expression of the keratin-4, -13, -14, -17 and transglutaminase 3 genes during the development of oral squamous cell carcinoma from leukoplakia
- Ist Teil von
- Oral oncology, 2005-07, Vol.41 (6), p.607-613
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To identify differentially expressed genes during the development of oral malignancy, differential display, northern blotting, reverse transcription–polymerase chain reaction (RT–PCR) and immunohistochemical analyses were undertaken using oral squamous cell carcinoma (OSCC) and leukoplakia tissues. Significantly higher levels of keratin ( Ker)-14 and -17 mRNAs, combined with lower levels of Ker-4, Ker-13 and transglutaminase 3 ( TG-3) transcripts, were observed in OSCC and severely dysplastic tissues, whereas this expression profile was reversed in hyperplasia and in mild to moderate dysplasia. The expression of Ker-4 and Ker-13 was elevated in density-arrested OSCC cell lines (Ca9-22, HSC-2, -3 and -4) but the expression of Ker-17 mRNA was elevated in these cells, regardless of the growth conditions. In addition, Ker-4 and Ker-13 proteins were predominantly expressed in moderate dysplasia and hyperplasia, whereas Ker-17 was markedly expressed in OSCC tissues. The expression patterns of these genes could therefore be an important determinant of the manifestation of oral malignancy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1368-8375eISSN: 1879-0593DOI: 10.1016/j.oraloncology.2005.01.011Titel-ID: cdi_proquest_miscellaneous_67968241
- Format
- –
- Schlagworte
- Adult, Aged, Aged, 80 and over, Biological and medical sciences, Calcium-Binding Proteins - biosynthesis, Calcium-Binding Proteins - genetics, Carcinoma, Squamous Cell - metabolism, Differential display, Disease Progression, Female, Gene expression, Gene Expression Regulation, Neoplastic, Humans, Hyperplasia - metabolism, Immunoenzyme Techniques, Immunohistochemistry, Keratin-14, Keratin-4, -13, -14, -17, Keratins - biosynthesis, Keratins - genetics, Leukoplakia, Leukoplakia, Oral - metabolism, Leukoplakia, Oral - pathology, Male, Medical sciences, Middle Aged, Mouth Neoplasms - metabolism, Neoplasm Proteins - biosynthesis, Neoplasm Proteins - genetics, Oral squamous cell carcinoma, Otorhinolaryngology. Stomatology, Reverse Transcriptase Polymerase Chain Reaction - methods, RNA, Messenger - genetics, RNA, Neoplasm - genetics, Transglutaminase 3, Transglutaminases - biosynthesis, Transglutaminases - genetics, Tumor Cells, Cultured, Tumors, Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology