Science (American Association for the Advancement of Science), 2005-06, Vol.308 (5730), p.1915-1920
2005
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Details
- Autor(en) / Beteiligte
- Titel
- Community Proteomics of a Natural Microbial Biofilm
- Ist Teil von
- Science (American Association for the Advancement of Science), 2005-06, Vol.308 (5730), p.1915-1920
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2005
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Using genomic and mass spectrometry-based proteomic methods, we evaluated gene expression, identified key activities, and examined partitioning of metabolic functions in a natural acid mine drainage (AMD) microbial biofilm community. We detected 2033 proteins from the five most abundant species in the biofilm, including 48% of the predicted proteins from the dominant biofilm organism, Leptospirillum group II. Proteins involved in protein refolding and response to oxidative stress appeared to be highly expressed, which suggests that damage to biomolecules is a key challenge for survival. We validated and estimated the relative abundance and cellular localization of 357 unique and 215 conserved novel proteins and determined that one abundant novel protein is a cytochrome central to iron oxidation and AMD formation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1109070Titel-ID: cdi_proquest_miscellaneous_67965754
- Format
- –
- Schlagworte
- Algorithms, Amino acids, Amino Acids - metabolism, Analysis, Animal, plant and microbial ecology, Archaeal Proteins - analysis, Archaeal Proteins - chemistry, Bacteria - chemistry, Bacteria - genetics, Bacteria - metabolism, Bacterial Proteins - analysis, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - physiology, Biofilms, Biofilms - growth & development, Biological and medical sciences, Community Relations, Cytochromes, Cytochromes - analysis, Cytochromes - chemistry, Ecosystem, Environment, Fractions, Freshwater, Fundamental and applied biological sciences. Psychology, Gene Expression, Genes, Archaeal, Genes, Bacterial, Genetic aspects, Genome, Archaeal, Genome, Bacterial, Genomes, Genomics, Hydrogen-Ion Concentration, Iron - metabolism, Isoelectric Point, Mass Spectrometry, Microbial ecology, Microbial mats, Mining, Molecular Sequence Data, Operons, Oxidation, Oxidation-Reduction, Protein Biosynthesis, Protein Folding, Protein metabolism, Proteins, Proteome, Proteomes, Proteomics, Structure, Thermoplasmales - chemistry, Thermoplasmales - genetics, Thermoplasmales - metabolism, Various environments (extraatmospheric space, air, water)