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Application of lentivirus‐mediated RNAi in studying gene function in mammalian tooth development
Developmental dynamics, 2006-05, Vol.235 (5), p.1347-1357
Song, Yiqiang
Zhang, Zunyi
Yu, Xueyan
Yan, Minquan
Zhang, Xiaoyun
Gu, Shuping
Stuart, Thomas
Liu, Chao
Reiser, Jakob
Zhang, Yanding
Chen, Yiping
2006
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Song, Yiqiang
Zhang, Zunyi
Yu, Xueyan
Yan, Minquan
Zhang, Xiaoyun
Gu, Shuping
Stuart, Thomas
Liu, Chao
Reiser, Jakob
Zhang, Yanding
Chen, Yiping
Titel
Application of lentivirus‐mediated RNAi in studying gene function in mammalian tooth development
Ist Teil von
Developmental dynamics, 2006-05, Vol.235 (5), p.1347-1357
Ort / Verlag
New York: Wiley‐Liss, Inc
Erscheinungsjahr
2006
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
RNA interference (RNAi) has recently become a powerful tool to silence gene expression in mammalian cells, but its application in assessing gene function in mammalian developing organs remains highly limited. Here we describe several unique developmental properties of the mouse molar germ. Embryonic molar mesenchyme, but not the incisor mesenchyme, once dissociated into single cell suspension and re‐aggregated, retains its odontogenic potential, the capability of a tissue to instruct an adjacent tissue to initiate tooth formation. Dissociated molar mesenchymal cells, even after being plated in cell culture, retain odontogenic competence, the capability of a tissue to respond to odontogenic signals and to support tooth formation. Most interestingly, while dissociated epithelial and mesenchymal cells of molar tooth germ are mixed and re‐aggregated, the epithelial cells are able to sort out from the mesenchymal cells and organize into a well‐defined dental epithelial structure, leading to the formation of a well‐differentiated tooth organ after sub‐renal culture. These unique molar developmental properties allow us to develop a strategy using a lentivirus‐mediated RNAi approach to silence gene expression in dental mesenchymal cells and assess gene function in tooth development. We show that knockdown of Msx1 or Dlx2 expression in the dental mesenchyme faithfully recapitulates the tooth phenotype of their targeted mutant mice. Silencing of Barx1 expression in the dental mesenchyme causes an arrest of tooth development at the bud stage, demonstrating a crucial role for Barx1 in tooth formation. Our studies have established a reliable and rapid assay that would permit large‐scale analysis of gene function in mammalian tooth development. Developmental Dynamics 235:1334–1344, 2006. © 2006 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 1058-8388
eISSN: 1097-0177
DOI: 10.1002/dvdy.20706
Titel-ID: cdi_proquest_miscellaneous_67911046
Format
–
Schlagworte
Animals
,
Barx1
,
Cell Line
,
Dlx
,
Gene Expression Regulation, Developmental
,
gene function
,
Gene Silencing
,
Genetic Vectors
,
Humans
,
lentivirus
,
Lentivirus - genetics
,
Mice
,
Mice, Knockout
,
Msx1
,
RNA Interference
,
RNA, Small Interfering - genetics
,
RNAi
,
Tooth - embryology
,
Tooth - metabolism
,
Tooth - virology
,
tooth development
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