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Accumulation of hydrogen peroxide is an early and crucial step for paclitaxel‐induced cancer cell death both in vitro and in vivo
International journal of cancer, 2006-07, Vol.119 (1), p.41-48
Alexandre, Jérôme
Batteux, Frédéric
Nicco, Carole
Chéreau, Christiane
Laurent, Alexis
Guillevin, Loïc
Weill, Bernard
Goldwasser, François
2006
Details
Autor(en) / Beteiligte
Alexandre, Jérôme
Batteux, Frédéric
Nicco, Carole
Chéreau, Christiane
Laurent, Alexis
Guillevin, Loïc
Weill, Bernard
Goldwasser, François
Titel
Accumulation of hydrogen peroxide is an early and crucial step for paclitaxel‐induced cancer cell death both in vitro and in vivo
Ist Teil von
International journal of cancer, 2006-07, Vol.119 (1), p.41-48
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2006
Link zum Volltext
Quelle
Wiley Online Library All Journals
Beschreibungen/Notizen
Intracellular events following paclitaxel binding to microtubules that lead to cell death remain poorly understood. Because reactive oxygen species (ROS) are involved in the cytotoxicity of anticancer agents acting through independent molecular targets, we explored the role of ROS in paclitaxel cytotoxicity. Within 15 min after in vitro exposure of A549 human lung cancer cells to paclitaxel, a concentration‐dependent intracellular increase in O°2− and H2O2 levels was detected by spectrofluorometry. Addition of N‐acetylcysteine (NAC) or glutathione, two H2O2 scavenger, induced a 4‐fold increase in paclitaxel IC50. Delaying NAC co‐incubation by 4 hr, resulted in a 3‐fold reduction in cell protection. The glutathione synthesis inhibitor, buthionine sulfoximine significantly increased paclitaxel cytotoxicity and H2O2 accumulation, but did not modify O°2− levels. Co‐incubation with diphenylene iodonium suggested that paclitaxel induced‐O°2− production was in part associated with increased activity of cytoplasmic NADPH oxidase. Concomitant treatment with inhibitors of caspases 3 and 8 increased cell survival but did not prevent the early accumulation of H2O2. To evaluate the role of ROS in paclitaxel antitumoral activity, mice were injected with LLC1 lung cancer cells and treated with paclitaxel i.p. and/or NAC. The antitumoral activity of paclitaxel in mice was abolished by NAC. In conclusion, the accumulation of H2O2 is an early and crucial step for paclitaxel‐induced cancer cell death before the commitment of the cells into apoptosis. These results suggest that ROS participate in vitro and in vivo to paclitaxel cytotoxicity. © 2006 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0020-7136
eISSN: 1097-0215
DOI: 10.1002/ijc.21685
Titel-ID: cdi_proquest_miscellaneous_67895073
Format
–
Schlagworte
Acetylcysteine - pharmacology
,
Antineoplastic agents
,
Antineoplastic Agents, Phytogenic - pharmacology
,
Apoptosis - drug effects
,
Biological and medical sciences
,
Cell Line, Tumor
,
Chemotherapy
,
Free Radical Scavengers - pharmacology
,
Glutathione - pharmacology
,
Humans
,
hydrogen peroxide
,
Hydrogen Peroxide - metabolism
,
Lung Neoplasms - drug therapy
,
Lung Neoplasms - metabolism
,
Medical sciences
,
NADPH oxidase
,
N‐acetylcysteine
,
paclitaxel
,
Paclitaxel - pharmacology
,
Pharmacology. Drug treatments
,
reactive oxygen species
,
Reactive Oxygen Species - metabolism
,
Spectrometry, Fluorescence
,
Tumors
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