Details

Autor(en) / Beteiligte

• Řehulka, Pavel
• Popkov, Alexander
• Nádvorník, Milan
• Planeta, Josef
• Mazanec, Karel
• Chmelík, Josef

Titel

Off-line combination of reversed-phase liquid chromatography and laser desorption/ionization time-of-flight mass spectrometry with seamless post-source decay fragment ion analysis for characterization of square-planar nickel(II) complexes

Ist Teil von

• Journal of mass spectrometry., 2006-04, Vol.41 (4), p.448-453

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

2006

Quelle

Wiley Online Library

Beschreibungen/Notizen

• Characterization of square‐planar nickel(II) complexes of the Schiff base of (S)‐N‐benzylproline (2‐benzoylphenyl)amide and various amino acids that are used as efficient α‐amino acids synthons was carried out using laser desorption/ionization time‐of‐flight mass spectrometry (LDI‐TOF MS) in off‐line combination with liquid chromatography. A mixture of four square‐planar nickel(II) complexes was separated using reversed‐phase liquid chromatography (RPLC) and the separated fractions from the chromatographic run were spotted on the metal target directly from the column outlet using a lab‐made sample deposition device. The separated fractions were then analyzed by LDI‐TOF MS. Seamless postsource decay (sPSD) fragment ion analysis was used for their structural characterization, which made possible the confirmation of expected chemical structures of the analyzed compounds. The off‐line combination of the separation by RPLC and analysis by LDI‐TOF MS allowed successful separation, sensitive detection and structure elucidation of the square‐planar nickel(II) complexes. Copyright © 2006 John Wiley & Sons, Ltd.