Details

Autor(en) / Beteiligte

• Earl, Elizabeth R.
• Waterston, Mary M.
• Aughey, Elizabeth
• Harvey, Michael J.A.
• Matschke, Christian
• Colston, Angela
• Ferro, Valerie A.

Titel

Evaluation of two GnRH-I based vaccine formulations on the testes function of entire Suffolk cross ram lambs

Ist Teil von

• Vaccine, 2006-04, Vol.24 (16), p.3172-3183

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2006

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• A modified GnRH peptide (CHWSYGLRPG-NH 2) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide- co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis.