Vaccine, 2006-04, Vol.24 (16), p.3191-3202
2006
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Details
- Autor(en) / Beteiligte
- Titel
- Delivery of tumor associated antigens to antigen presenting cells using penetratin induces potent immune responses
- Ist Teil von
- Vaccine, 2006-04, Vol.24 (16), p.3191-3202
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cytoplasmic delivery of proteins or CTL epitopes is crucial for the presentation of antigen for the generation of CTL. We previously described the use of the 16-amino acid peptide penetratin from the Drosophila Antennapedia domain (Int) to transport CTL epitopes into cells. Here we show that, Int, incorporating MUC1 CTL epitopes in tandem is able to facilitate their rapid uptake by macrophages and dendritic cells (DC) in an energy-dependent endocytic pathway. We also demonstrate for the first time that Int conjugated proteins are also able to be efficiently taken up by DC. Furthermore, C57BL/6 and HLA-A2 transgenic mice immunized with the Int-peptides or Int-proteins induce strong IFN-γ secreting T cells and weak IgG1 antibodies. Immunized C57BL/6 mice were protected against the growth of a MUC1 + tumor cell line.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2006.01.032Titel-ID: cdi_proquest_miscellaneous_67807377
- Format
- –
- Schlagworte
- Amino acids, Animals, Antennapedia, Antibodies, Neoplasm - blood, Antigen Presentation, Applied microbiology, Biological and medical sciences, Cancer Vaccines - immunology, Carrier Proteins - administration & dosage, Carrier Proteins - genetics, Carrier Proteins - pharmacology, Cell Line, Tumor, Cytoplasm, Cytotoxicity, Dendritic Cells - immunology, Dendritic Cells - metabolism, Enzyme-Linked Immunosorbent Assay, Epitopes, T-Lymphocyte - immunology, Epitopes, T-Lymphocyte - metabolism, Fundamental and applied biological sciences. Psychology, HLA-A2 Antigen - genetics, Immunization, Immunoglobulin G - blood, Influenza, Interferon-gamma - biosynthesis, Lymphocytes, Macrophages - immunology, Macrophages - metabolism, Measles, Membrane translocating peptide, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microbiology, Microscopy, Fluorescence, MUC1, Mucin-1 - immunology, Mucin-1 - metabolism, Neoplasms, Experimental - immunology, Penetratin, Peptides, Proteins, Recombinant Fusion Proteins - administration & dosage, Recombinant Fusion Proteins - immunology, Recombinant Fusion Proteins - metabolism, Studies, T-Lymphocytes - immunology, Trojan, Vaccines, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)