Journal of biochemistry (Tokyo), 2006-03, Vol.139 (3), p.607-614
2006
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- Autor(en) / Beteiligte
- Titel
- Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type 2
- Ist Teil von
- Journal of biochemistry (Tokyo), 2006-03, Vol.139 (3), p.607-614
- Ort / Verlag
- England: Oxford University Press
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The interaction between cell surface receptors and the envelope glycoprotein (EGP) on the viral membrane surface is the initial step of Dengue virus infection. To understand the host range, tissue tropism, and virulence of this pathogen, it is critical to elucidate the molecular mechanisms of the interaction of EGP with receptor molecules. Here, using a TLC/virus-binding assay, we isolated and characterized a carbohydrate molecule on mammalian cell surfaces that is recognized by dengue virus type 2 (DEN2). Structural determination by immunochemical methods showed that the carbohydrate structure of the purified glycosphingolipid was neolactotetraosylceramide (nLc₄Cer). This glycosphingolipid was expressed on the cell surface of susceptible cells, such as human erythroleukemia K562 and baby hamster kidney BHK-21. All serotypes of DEN viruses, DEN1 to DEN4, reacted with nLc₄Cer, and the non-reducing terminal disaccharide residue Gal{szligbeta}1-4GlcNAc{szligbeta}1- was found to be a critical determinant for the binding of DEN2. Chemically synthesized derivatives carrying multiple carbohydrate residues of nLc₄, but not nLc₄ oligosaccharide, inhibited DEN2 infection of BHK-21 cells. These findings strongly suggested that multivalent nLc₄ oligosaccharide could act as a competitive inhibitor against the binding of DEN2 to the host cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-924XeISSN: 1756-2651DOI: 10.1093/jb/mvj067Titel-ID: cdi_proquest_miscellaneous_67800912
- Format
- –
- Schlagworte
- Animals, Ar4Cer, Brain - metabolism, Carbohydrate Sequence, Cattle, Cell Line, Cricetinae, DEN2, dendrimer, Dendrimers - chemistry, Dendrimers - metabolism, dengue virus, Dengue Virus - metabolism, Dengue virus type 2, DMEM, Dulbecco's modified Eagle's medium, EGP, envelope glycoprotein, FBS, fetal bovine serum, FFU, focus forming unit, Gal, galactose, GalNAc, GalNAcβ1-4Galβ1-4Glcβ1-1′Cer, GalNAcβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1′Cer, Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1′Cer, Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1′Cer, Galβ1-4Glcβ1-1′Cer, Gg3Cer, Gg4Cer, Glc, GlcNAc, glucose, glycosphingolipid, Glycosphingolipids - chemistry, Glycosphingolipids - metabolism, heparan sulfate, horse radish peroxidase, HRP, Humans, K562 Cells, LacCer, lacto-N-neotetraose, Molecular Sequence Data, N-acetylgalactosamine, N-acetylglucosamine, nLc4, nLc4Cer, thin layer chromatography, TLC