Details

Autor(en) / Beteiligte

• Zariffard, M. Reza
• Novak, Richard M
• Lurain, Nell
• Sha, Beverly E
• Graham, Parrie
• Spear, Gregory T

Titel

Induction of Tumor Necrosis Factor–α Secretion and Toll-Like Receptor 2 and 4 mRNA Expression by Genital Mucosal Fluids from Women with Bacterial Vaginosis

Ist Teil von

• The Journal of infectious diseases, 2005-06, Vol.191 (11), p.1913-1921

Ort / Verlag

Chicago, IL: The University of Chicago Press

Erscheinungsjahr

2005

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• BackgroundBacterial vaginosis (BV) is associated with complications of pregnancy and increased susceptibility to human immunodeficiency virus (HIV) sexual transmission MethodsThe ability of genital mucosal fluids from women with BV and of microbial flora associated with BV to induce tumor necrosis factor (TNF)–α secretion and Toll-like receptor (TLR) 2 and TLR4 mRNA expression was assessed ResultsPrimary peripheral-blood mononuclear cells and THP-1 monocytic cells secreted TNF-α in response to cervicovaginal lavage (CVL) samples from women with BV. Mycoplasma hominis and Gardnerella vaginalis also stimulated TNF-α secretion. Strikingly, CVL samples from women with BV induced up to 60-fold increases in TLR4 mRNA expression, compared with CVL samples from women without BV and with bacteria not associated with BV. Anti–TNF-α antibody blocked increases in TLR4 mRNA expression induced by CVL samples from women with BV, indicating that TNF-α plays a critical role in induction of TLR4. Both TLR2 and TLR4 mRNA expression were ∼60-fold higher in cells isolated from the lumen of the genital tract than in cervical mucosal tissue, but lumen TLR mRNA levels did not change significantly after BV treatment ConclusionsThese experiments show that genital mucosal fluids and certain bacteria from women with BV stimulate TNF-α secretion and TLR4 mRNA expression, suggesting mechanisms whereby BV affects pregnancy and HIV transmission