Details

Autor(en) / Beteiligte

• SUZUKI, Fumika
• HASHIMOTO, Ken
• SAKAGAMI, Hiroshi
• KIKUCHI, Hirotaka
• NISHIKAWA, Hirofumi
• MATSUMOTO, Hisatoshi
• SHIMADA, Jun
• KAWASE, Masami
• SUNAGA, Katsuyoshi
• TSUDA, Tadashi
• SATOH, Kazue

Titel

Induction of Tumor-specific Cytotoxicity and Apoptosis by Doxorubicin

Ist Teil von

• Anticancer research, 2005-03, Vol.25 (2A), p.887-893

Ort / Verlag

Attiki: International Institute of Anticancer Research

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• Doxorubicin (adriamycin), an anthracycline antibiotic, showed higher cytotoxic activity against human tumor cell lines (oral squamous cell carcinoma HSC-2, HSC-3, submandibular gland carcinoma HSG, promyelocytic leukemia HL-60) than against normal human cells (gingival fibroblast HGF, pulp cell HPC, periodontal ligament fibroblast HPLF). Doxorubicin activated caspases 3, 8 and 9 in both HSC-2 and HL-60 cells, but induced internucleosomal DNA fragmentation only in HL-60 cells. Western blot analysis showed that doxorubicin did not significantly change the intracellular concentration of Bcl-2, Bax and Bad in HL-60 cells. Real-time PCR analysis showed that HPC cells expressed the highest amount of mdr1 mRNA, followed by HSC-2 > HGF > HSC-3 > HPLF > HSG > HL-60. ESR spectroscopy showed that doxorubicin produced no discernible radical under alkaline conditions (pH 7.4 to 10.5) except at pH 12.5, and it did not scavenge O 2 - , NO and DPPH radicals. The present study demonstrates that doxorubicin induces the tumor-specific cytotoxicity and some, but not all, apoptosis markers possibly by a radical-independent mechanism, and that mdr1 expression in the tumor cells is not related to the tumor specificity of doxorubicin.