Current microbiology, 2006-04, Vol.52 (4), p.243-248
2006
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- Autor(en) / Beteiligte
- Titel
- Cloning, sequencing and characterization of a novel phosphatase gene, phoI, from soil bacterium Enterobacter sp. 4
- Ist Teil von
- Current microbiology, 2006-04, Vol.52 (4), p.243-248
- Ort / Verlag
- United States: Springer Nature B.V
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% identity and 62.4% homology to the hel gene from Heamophilus influenzae, which encoded an outer membrane protein (P4). The optimum pH and temperature for phosphatase activity were pH 5.5 and 40 degrees C, respectively. Its specific activity on rho-nitrophenyl phosphatate was 70 U/mg at pH 5.5 and 40 degrees C. Enzyme activity was inhibited by Al3+, EDTA, and DTT, but fivefold activated by Cu2+ ion (350 U/mg). PhoI showed a strong synergistic effect when used with a purified E. coli phytase, AppA, to estimate combination effects.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0343-8651eISSN: 1432-0991DOI: 10.1007/s00284-005-4467-zTitel-ID: cdi_proquest_miscellaneous_67780326
- Format
- –
- Schlagworte
- 6-Phytase - metabolism, Acid phosphatase, Acid Phosphatase - genetics, Acid Phosphatase - metabolism, Aluminum, Amino Acid Sequence, Amino acids, Bacteria, Bacterial Outer Membrane Proteins - genetics, Bacteriology, Cloning, Cloning, Molecular, Copper, Dithiothreitol, E coli, Enterobacter, Enterobacter - enzymology, Enzymatic activity, Enzyme activity, Enzymes, Escherichia coli, Escherichia coli - enzymology, Escherichia coli Proteins - metabolism, Esterases - genetics, Ethylenediaminetetraacetic acids, Hel protein, Homology, Hydrogen-Ion Concentration, Lipoproteins - genetics, Membrane proteins, Molecular Sequence Data, Open reading frames, pH effects, Phosphates - metabolism, Phytase, Phytic Acid - metabolism, Proteins, Sequence Alignment, Soil bacteria, Soil microorganisms, Substrate Specificity, Synergistic effect, Temperature