Details

Autor(en) / Beteiligte

• Wardrope, Caroline
• Mowat, Christopher G.
• Walkinshaw, Malcolm D.
• Reid, Graeme A.
• Chapman, Stephen K.

Titel

Fumarate reductase: Structural and mechanistic insights from the catalytic reduction of 2-methylfumarate

Ist Teil von

• FEBS letters, 2006-03, Vol.580 (6), p.1677-1680

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2006

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The soluble fumarate reductase (FR) from Shewanella frigidimarina can catalyse the reduction of 2-methylfumarate with a k cat of 9.0 s −1 and a K M of 32 μM. This produces the chiral molecule 2-methylsuccinate. Here, we present the structure of FR to a resolution of 1.5 Å with 2-methylfumarate bound at the active site. The mode of binding of 2-methylfumarate allows us to predict the stereochemistry of the product as ( S)-2-methylsuccinate. To test this prediction we have analysed the product stereochemistry by circular dichroism spectroscopy and confirmed the production of ( S)-2-methylsuccinate.