Science (American Association for the Advancement of Science), 2009-09, Vol.325 (5946), p.1394-1398
2009
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Details
- Autor(en) / Beteiligte
- Titel
- RNA-Binding Protein NANOS2 Is Required to Maintain Murine Spermatogonial Stem Cells
- Ist Teil von
- Science (American Association for the Advancement of Science), 2009-09, Vol.325 (5946), p.1394-1398
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2009
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Stem cells give rise to differentiated cell types but also preserve their undifferentiated state through cell self-renewal. With the use of transgenic mice, we found that the RNA-binding protein NANOS2 is essential for maintaining spermatogonial stem cells. Lineage-tracing analyses revealed that undifferentiated spermatogonia expressing Nanos2 self-renew and generate the entire spermatogenic cell lineage. Conditional disruption of postnatal Nanos2 depleted spermatogonial stem cell reserves, whereas mouse testes in which Nanos2 had been overexpressed accumulated spermatogonia with undifferentiated, stem cell-like properties. Thus, NANOS2 is a key stem cell regulator that is expressed in self-renewing spermatogonial stem cells and maintains the stem cell state during murine spermatogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1172645Titel-ID: cdi_proquest_miscellaneous_67648623
- Format
- –
- Schlagworte
- Animals, Basic Helix-Loop-Helix Transcription Factors - metabolism, Biological and medical sciences, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cell Differentiation, Cell differentiation, maturation, development, hematopoiesis, Cell Lineage, Cell physiology, Cellular biology, Fundamental and applied biological sciences. Psychology, Gene Knockout Techniques, Glial Cell Line-Derived Neurotrophic Factor Receptors - metabolism, Kruppel-Like Transcription Factors - metabolism, Male, Males, Mice, Mice, Transgenic, Molecular and cellular biology, Nerve Tissue Proteins - metabolism, Promyelocytic Leukemia Zinc Finger Protein, Reproductive system, RNA-Binding Proteins - metabolism, RNA-protein interactions, Rodents, Spermatogenesis, Spermatogonia - cytology, Spermatogonia - metabolism, Stem cells, Stem Cells - cytology, Stem Cells - metabolism, Testis - cytology, Testis - metabolism, Zinc Fingers