Details

Autor(en) / Beteiligte

• Perera, Omaththage P.
• Valles, Steven M.
• Green, Terry B.
• White, Susan
• Strong, Charles A.
• Becnel, James J.

Titel

Molecular analysis of an occlusion body protein from Culex nigripalpus nucleopolyhedrovirus (CuniNPV)

Ist Teil von

• Journal of invertebrate pathology, 2006, Vol.91 (1), p.35-42

Ort / Verlag

Amsterdam: Elsevier Inc

Erscheinungsjahr

2006

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• CUN085 is an occlusion body (OB) protein from the nucleopolyhedrovirus of Culex nigripalpus (CuniNPV). SDS–PAGE analysis indicated that the CuniNPV OB protein is about 3 times the size (∼90 kDa) of characterized nucleopolyhedrovirus (NPVs) and granulovirus OB proteins. Rapid amplification of cDNA ends (RACE), RNase protection assay, real-time PCR, and protein sequencing were used to characterize CUN085 from CuniNPV. RACE data revealed that the transcriptional start and termination sites for the CUN085 gene yielded a polypeptide comprised of 822 amino acids indicating that translation initiates within a larger 882 amino acid open reading frame that was originally predicted from the CuniNPV genome sequence. Transcription of CUN085 started at a consensus baculovirus late transcription start site TAAG at nucleotide position 75433 of the CuniNPV genome sequence. RNase protection assays and quantitative real-time PCR show that the CUN085 transcript is first detected in mosquito larvae at approximately 6 h after infection with CuniNPV and its prevalence increased progressively over the subsequent 18 h.