Details

Autor(en) / Beteiligte

• Chen, Yi-Guang
• Xiao, Huai-Dong
• Tang, Shu-Kun
• Zhang, Yu-Qin
• Borrathybay, Entomack
• Cui, Xiao-Long
• Li, Wen-Jun
• Liu, Yan-Qi

Titel

Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone

Ist Teil von

• Antonie van Leeuwenhoek, 2009-10, Vol.96 (3), p.259-266

Ort / Verlag

Dordrecht: Dordrecht : Springer Netherlands

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• A pale yellow-colored, moderately halophilic, Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped, motile, aerobic bacterium, designated strain JSM 073008T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow with 1-20% (w/v) total salts (optimum, 5-10%), at pH 6.0-10.0 (optimum, pH 7.5) and 10-40°C (optimum, 25-30°C). The major cellular fatty acids were C₁₆:₀, C₁₆:₁ ω7c/iso-C₁₅:₀ 2-OH and C₁₈:₁ ω7c. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The predominant respiratory quinone was Q-8 and the genomic DNA G + C content was 47.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 073008T should be assigned to the genus Alteromonas, being most closely related to Alteromonas hispanica F-32T (sequence similarity 96.9%), followed by Alteromonas genovensis LMG 24078T (96.6%) and Alteromonas litorea TF-22T (96.4%). The sequence similarities between the novel isolate and the type strains of other recognized Alteromonas species ranged from 95.9% (with Alteromonas stellipolaris ANT 69aT) to 94.5% (with Alteromonas simiduii BCRC 17572T). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 073008T represents a new species of the genus Alteromonas, for which the name Alteromonas halophila sp. nov. is proposed. The type strain is JSM 073008T (=CCTCC AA 207035T = KCTC 22164T).