The Journal of immunology (1950), 2009-09, Vol.183 (5), p.3118-3129
2009
Details
- Autor(en) / Beteiligte
- Titel
- TCR Repertoire and Foxp3 Expression Define Functionally Distinct Subsets of CD4+ Regulatory T Cells
- Ist Teil von
- The Journal of immunology (1950), 2009-09, Vol.183 (5), p.3118-3129
- Ort / Verlag
- United States: Am Assoc Immnol
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Despite extensive research efforts to characterize peripheral regulatory T (T(reg)) cells expressing transcription factor Foxp3, their subset complexity, phenotypic characteristics, TCR repertoire and Ag specificities remain ambiguous. In this study, we identify and define two subsets of peripheral T(reg) cells differing in Foxp3 expression level and TCR repertoires. T(reg) cells expressing a high level of Foxp3 and TCRs not used by naive CD4(+) T cells present a stable suppressor phenotype and dominate the peripheral T(reg) population in unmanipulated mice. The second T(reg) subset, expressing a lower level of Foxp3 and using TCRs shared with naive CD4(+) T cells constitutes a small fraction of all T(reg) cells in unmanipulated mice and enriches T(reg) population with the same Ag specificities as expressed by activated/effector T cells. This T(reg) subset undergoes extensive expansion during response to Ag when it becomes a major population of Ag-specific T(reg) cells. Thus, T(reg) cells expressing TCRs shared with naive CD4(+) T cells have a flexible phenotype and may down-regulate Foxp3 expression which may restore immune balance at the conclusion of immune response or convert these cells to effector T cells producing inflammatory cytokines.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1767eISSN: 1550-6606DOI: 10.4049/jimmunol.0900514Titel-ID: cdi_proquest_miscellaneous_67593643
- Format
- –
- Schlagworte
- Adoptive Transfer, Animals, CD4 Antigens - administration & dosage, CD4 Antigens - biosynthesis, Cell Differentiation - genetics, Cell Differentiation - immunology, Cells, Cultured, Coculture Techniques, Down-Regulation - genetics, Down-Regulation - immunology, Forkhead Transcription Factors - antagonists & inhibitors, Forkhead Transcription Factors - biosynthesis, Forkhead Transcription Factors - genetics, Gene Expression Regulation - immunology, Green Fluorescent Proteins - biosynthesis, Green Fluorescent Proteins - genetics, Immunity, Innate - genetics, Immunophenotyping, Mice, Mice, Inbred C57BL, Mice, Transgenic, Models, Immunological, Receptors, Antigen, T-Cell - biosynthesis, Receptors, Antigen, T-Cell - genetics, Receptors, Antigen, T-Cell - physiology, Resting Phase, Cell Cycle - genetics, Resting Phase, Cell Cycle - immunology, T-Lymphocyte Subsets - immunology, T-Lymphocyte Subsets - metabolism, T-Lymphocyte Subsets - transplantation, T-Lymphocytes, Regulatory - immunology, T-Lymphocytes, Regulatory - metabolism, T-Lymphocytes, Regulatory - transplantation, Thymus Gland - cytology, Thymus Gland - immunology, Thymus Gland - metabolism