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Meiotic Arrest In Vitro by Phosphodiesterase 3-Inhibitor Enhances Maturation Capacity of Human Oocytes and Allows Subsequent Embryonic Development
Ist Teil von
Biology of reproduction, 2006-01, Vol.74 (1), p.177-184
Ort / Verlag
Madison, WI: Society for the Study of Reproduction
Erscheinungsjahr
2006
Quelle
MEDLINE
Beschreibungen/Notizen
Controlling nuclear maturation during oocyte culture might improve nuclear-cytoplasmic maturation synchrony. We aimed to evaluate
the quality of in vitro-matured, germinal vesicle (GV)-stage human oocytes following a prematuration culture (PMC) with a
meiotic arrester, phosphodiesterase 3-inhibitor (PDE3-I). Follicles (diameter, 6â12 mm) were retrieved 34â36 h post-hCG administration
from informed, consenting patients who had undergone controlled ovarian stimulation. Cumulus-enclosed oocytes (CEOs) presenting
moderate expansion or full compaction were placed in PMC with the PDE3-I, Org9935, for 24 or 48 h. Subsequently, oocytes were
removed from PMC, denuded of cumulus cells, matured in vitro, and fertilized, and the resulting embryos were cultured. In
the presence of PDE3-I, approximately 98% of the oocytes were arrested at the GV stage. Following PDE3-I removal, oocytes
acquired a higher maturation rate than oocytes that were immediately denuded of cumulus cells after retrieval and in vitro
matured (67% vs. 46%, P = 0.01). In controls, immature CEOs retrieved with moderate expansion reached higher maturation rates compared to fully compacted
CEOs, but in PMC groups, high values of maturation were achieved for both morphological classes of CEOs. No effect of PMC
on fertilization was observed. A 24-h PMC period proved to be the most effective in preserving embryonic integrity. Similar
proportions of nuclear abnormalities were observed in embryos of all in vitro groups. In summary, PMC with the specific PDE3-I
had a beneficial effect on human CEOs by enhancing maturation, benefiting mainly the fully compacted CEOs. This resulted in
an increased yield of mature oocytes available for insemination without compromising embryonic development. These results
suggest that applying an inhibitor to control the rate of nuclear maturity by regulating intraoocyte PDE3 activity may allow
the synchronization of nuclear and ooplasmic maturation.
Abstract
Regulating intra-oocyte phosphodiesterase type 3 activity in vitro may allow the synchronization of nuclear and ooplasmic
maturation of human oocytes.