Details

Autor(en) / Beteiligte

• Gutensohn, K.
• Magens, M.
• Krüger, W.
• Kröger, N.
• Kühnl, P.

Titel

Comparison of flow cytometry vs. a haematology cell analyser-based method to guide the optimal time-point for peripheral blood stem cell apheresis

Ist Teil von

• Vox sanguinis, 2006-01, Vol.90 (1), p.53-58

Ort / Verlag

Oxford, UK: Blackwell Science Ltd

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• Background and Objectives  For timing the onset of apheresis, parameters obtained by flow cytometry and by a haematological cell analyser were compared. Materials and Methods  Haematopoietic cell counts (n = 159) were performed by two different methods; CD34 analyses by flow cytometry, immature myeloid information (IMI) and human progenitor cell counts (HPC) by a haematological cell analyser. Results  Comparing the IMI total results with CD34+ analyses (n = 159) revealed a correlation of r = 0·46 (P < 0·05). Similar results were obtained for HPC (r = 0·44; P < 0·05). Conclusion  The haematology analyser‐based method does not allow the precise determination of absolute haematopoietic stem cell numbers and is thus not able to replace flow cytometry for the monitoring of peripheral blood stem cell counts.