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Induction of Apoptosis in Human Colon Cancer HCT116 Cells Treated with an Extract of the Plant Product, Chios Mastic Gum
Ist Teil von
In vivo (Athens), 2005-01, Vol.19 (1), p.93-102
Ort / Verlag
Greece: International Institute of Anticancer Research
Erscheinungsjahr
2005
Quelle
MEDLINE
Beschreibungen/Notizen
A hexane extract of the plant product Chios mastic gum (He-CMG) is demonstrated to kill human colon cancer cells in vitro
via the process of anoikis. Specifically, the sequence of events includes He-CMG-induced G1-arrest of the cells, detachment
of the cells from the substrate and subsequent apoptosis. Anoikis is dependent on the concentration and duration of treatment
with He-CMG. Presence of the pan-caspase inhibitor, Z-VAD-fmk, did not prevent cell detachment, but it did prevent apoptosis
of the detached cells indicating that the process of cell detachment, but not apoptosis, is independent of caspase activation.
He-CMG-induced apoptosis is associated with activation of the initiator caspases-8, and -9 and the effector caspase-3. Caspases
are activated in cells at a relatively long time after detachment, and caspase-3 activation may require caspase-8 or caspase-9
activation, as determined by using HCT116 isogenic clones impaired in apoptosis mechanisms that involve these two caspases.
Finally, electron microscopy observations indicated a time-dependent appearance of morphological features both typical and
non-typical of apoptosis in cells treated with He-CMG for various periods of time. Taken together, the results demonstrated
that He-CMG induces apoptosis in HCT116 cells and, therefore, further in vivo and in vitro studies of the anticancer activities
of this plant product are warranted.