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Elevated nuclear maspin expression is associated with microsatellite instability and high tumour grade in colorectal cancer
The Journal of pathology, 2005-04, Vol.205 (5), p.606-614
Bettstetter, Marcus
Woenckhaus, Matthias
Wild, Peter J
Rümmele, Petra
Blaszyk, Hagen
Hartmann, Arndt
Hofstädter, Ferdinand
Dietmaier, Wolfgang
2005
Details
Autor(en) / Beteiligte
Bettstetter, Marcus
Woenckhaus, Matthias
Wild, Peter J
Rümmele, Petra
Blaszyk, Hagen
Hartmann, Arndt
Hofstädter, Ferdinand
Dietmaier, Wolfgang
Titel
Elevated nuclear maspin expression is associated with microsatellite instability and high tumour grade in colorectal cancer
Ist Teil von
The Journal of pathology, 2005-04, Vol.205 (5), p.606-614
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2005
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Maspin, a member of the serpin family, has been reported to suppress metastasis and angiogenesis in breast and prostate cancers. Overexpression of maspin was associated with adverse prognostic features in several other tumours. In this study, expression of maspin was analysed in 41 colorectal carcinomas with high‐frequency microsatellite instability (MSI‐H) and 159 microsatellite stable colorectal cancers (MSS/MSI‐L) by immunohistochemistry (IHC) and partly by relative quantitative real‐time RT‐PCR and western blot analyses. Significant upregulation of maspin expression was found in MSI‐H tumours compared to both MSS/MSI‐L tumours and matched benign colonic mucosa. Increased maspin expression was also found in three MSI‐H colon cancer cell lines, but not in three MSS colon cancer cell lines by RT‐PCR and western blot analyses. Regulation of maspin expression depended on promoter methylation as tissue specimens and cell lines expressing maspin showed unmethylated maspin promoters, whereas promoter hypermethylation was found in specimens with loss of maspin expression. Intense nuclear maspin immunostaining was seen specifically in MSI‐H tumours (p = 0.013), de‐differentiated tumours (p = 0.006), and at the invasion front. These findings provide new insights into the role of maspin in colorectal cancer progression and may be useful for diagnosis and treatment strategies. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Sprache
Englisch
Identifikatoren
ISSN: 0022-3417
eISSN: 1096-9896
DOI: 10.1002/path.1732
Titel-ID: cdi_proquest_miscellaneous_67550423
Format
–
Schlagworte
Biological and medical sciences
,
Biomarkers, Tumor - genetics
,
Biomarkers, Tumor - metabolism
,
Blotting, Western
,
Cell Nucleus - metabolism
,
colorectal cancer
,
Colorectal Neoplasms - genetics
,
Colorectal Neoplasms - metabolism
,
Colorectal Neoplasms - pathology
,
CpG Islands
,
Cytoplasm - metabolism
,
de-differentiation
,
DNA Methylation
,
DNA, Neoplasm - genetics
,
Gastroenterology. Liver. Pancreas. Abdomen
,
Genes, Tumor Suppressor
,
Humans
,
Investigative techniques, diagnostic techniques (general aspects)
,
maspin expression
,
Medical sciences
,
microsatellite instability
,
Microsatellite Repeats
,
Neoplasm Invasiveness
,
Neoplasm Proteins - metabolism
,
Neoplasm Staging
,
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
,
promoter methylation
,
Promoter Regions, Genetic
,
relative quantitative real-time PCR
,
Reverse Transcriptase Polymerase Chain Reaction - methods
,
RNA, Neoplasm - genetics
,
Serpins - genetics
,
Serpins - metabolism
,
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
,
Transcription, Genetic
,
Tumors
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