Peptides (New York, N.Y. : 1980), 2005-04, Vol.26 (4), p.581-587
2005
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Details
- Autor(en) / Beteiligte
- Titel
- Kenojeinin I, antimicrobial peptide isolated from the skin of the fermented skate, Raja kenojei
- Ist Teil von
- Peptides (New York, N.Y. : 1980), 2005-04, Vol.26 (4), p.581-587
- Ort / Verlag
- New York, NY: Elsevier Inc
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- An antimicrobial peptide was purified from fermented skate skin extract using the solid-phase extraction and separation on HPLC reversed-phase chromatography. Amino acid sequence of the purified peptide (Peak A) having an antimicrobial activity revealed the presence of many cationic residues of the total 28 amino acids. Its molecular mass was found to be 3059 Da. This result was in excellent agreement with the theoretical molecular mass calculated from the amino acid sequence. The synthetic kenojeinin I had inhibitory effects on B. subtilis (MIC, 12 μg/ml), E. coli (28 μg/ml), and S. cerevisiae (12 μg/ml). These results indicate that fermented skate skin is potentially antimicrobial.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0196-9781eISSN: 1873-5169DOI: 10.1016/j.peptides.2004.11.011Titel-ID: cdi_proquest_miscellaneous_67499944
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Anti-Bacterial Agents - chemistry, Anti-Bacterial Agents - isolation & purification, Anti-Bacterial Agents - pharmacology, Antifungal Agents - chemistry, Antifungal Agents - isolation & purification, Antifungal Agents - pharmacology, Antimicrobial Cationic Peptides - chemistry, Antimicrobial Cationic Peptides - isolation & purification, Antimicrobial Cationic Peptides - pharmacology, Antimicrobial peptide, Bacillus subtilis - drug effects, Biological and medical sciences, Escherichia coli - drug effects, Fundamental and applied biological sciences. Psychology, Kenojeinin I, Microbial Sensitivity Tests, Molecular Sequence Data, Molecular Weight, Peptides - isolation & purification, Peptides - pharmacology, Raja kenojei, Saccharomyces cerevisiae - drug effects, Skate skin, Skates (Fish), Skin - chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vertebrates: endocrinology