Details

Autor(en) / Beteiligte

• Remmerbach, Torsten W.
• Meyer‐Ebrecht, Dietrich
• Aach, Til
• Würflinger, Thomas
• Bell, Andre A.
• Schneider, Timna E.
• Nietzke, Nadja
• Frerich, Bernhard
• Böcking, Alfred

Titel

Toward a multimodal cell analysis of brush biopsies for the early detection of oral cancer

Ist Teil von

• Cancer, 2009-06, Vol.117 (3), p.228-235

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2009

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• BACKGROUND: This report describes what to the authors' knowledge is the first clinical application of semiautomated multimodal cell analysis (MMCA), a novel technique for the early detection of cancer for cases with a limited number of suspicious cells. In this clinical study, MMCA was applied to oral cancer diagnostics on brush biopsies. The MMCA approach was based on the sequential application of multiple stainings of identical, slide‐based cells and repeated relocalizations and measurements of their diagnostic features, resulting in multiparametric features of individual cells. Data integration of the variously stained cells increased diagnostic accuracy. The implementation of MMCA also enabled fully automatic, adaptive image preprocessing, including registration of multimodal images and segmentation of cell nuclei. METHODS: In a preliminary clinical trial, 47 slides from brush biopsies of suspicious oral lesions were analyzed. The final histologic diagnoses included 20 squamous cell carcinomas, 7 hyperkeratotic leukoplakias, and 20 lichen planus mucosae. RESULTS: The stepwise application of 2 additional approaches (morphology, DNA content, argyrophilic nucleolar organizer region counts) increased the specificity of conventional cytologic diagnosis from 92.6% to 100%. This feasibility study provided a proof of concept, demonstrating efficiency, robustness, and diagnostic accuracy on slide‐based cytologic specimens. CONCLUSIONS: The authors concluded that MMCA may become a sensitive and highly specific, objective, and reproducible adjuvant diagnostic tool for the identification of neoplastic changes in oral smears that contain only a few abnormal cells. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society. The authors investigated the clinical application of semiautomated multimodal cell analysis (MMCA), a novel technique for the early detection of oral cancer in patients who have a limited number of suspicious cells. The results indicated that semiautomated MMCA may become a sensitive and highly specific, objective, and reproducible adjuvant diagnostic tool for the identification of early neoplastic transformation in oral brush biopsies that contain only a few abnormal cells.