Details

Autor(en) / Beteiligte

• He, Xiao-Dong
• Hu, Shi-Lian
• Shen, Zuo-Jun
• Tao, Shao-Neng
• Dong, Lin
• Zhu, Yuan-Yuan
• Li, Ming

Titel

Chiral selectivity in differentiation of lung cancer A549 cell to vascular endothelial cells after drug resistance induced by D-or L-methotrexate enantiomers

Ist Teil von

• Zhong hua yi xue za zhi, 2009-03, Vol.89 (10), p.690-694

Ort / Verlag

China

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the chiral selectivity in vascular endothelial differentiation in drug resistant lung cancer cells induced by high-dose L- or D-methotrexate (MTX) enantiomer. Human lung cancer cells of the line A549 were co-cultured with high-dose (15 micromol/L) L- or D-MTX enantiomer so as to develop cancer cells resistant to MTX. MTT method was used to detect the drug resistant index. Flow cytometry was used to detect the expression of CD44, a transmembrane glycoprotein reflecting the migration ability of cells, CD31, a marker of vascular endothelium, and P-170 protein. Fifteen BALB/c nude mice were inoculated with the parent A549 cells, L-MTX-resistant A549 cells induced by L-MTX enantiomer, and D-MTX-resistant A549 cells induced by D-MTX enantiomer. Four weeks later the mice were killed to take out the tumor masses. Immunohistochemistry with CD34 staining was used to detect the microvascular density (MVD). The drug resistant index of the D-MTX induced drug resistant A549 cells was 20.1 +/- 2.3, significantly hi